Oligonucléotide

The oligonucléotide S are short sequences of Nucléotide S (ARN or DNA), simple-bit and long of a few tens of bases. They are in general obtained by chemical synthesis.

The oligonucléotides are often used like Sonde S in order to detect complementary DNA or ARN. As examples of use of the oligonucléotides one can quote:

• the chips with DNA
• technique of the Southern blot
• the technique FISH (Fluorescent In situ Hybridization)

The oligonucléotides composed of DNA (Déoxyoligonucléotide S) are often used for PCR (Polymerase Chain Reaction), proceeded making it possible to amplify any piece of DNA almost. In this case, the oligonucléotide is often indicated under the name of Amorce. This will generate a " cible" for a Polymerase which will be able to bind and to prolong the starter by the addition of nucleotides.
In l'" slang of the science" , the oligonucléotides are called oligos .